Coltivazione di cellule della papilla germinativa

[Lottatore]

Abstract TITLE: A simple and rapid method to isolate and culture follicular papillae from human scalp hair follicles AUTHORS (ALL): Magerl, Markus 1; Kauser, Söbia 1; Paus, Ralf 2; Tobin, Desmond 1 INSTITUTIONS (ALL): 1. Department of Biomedical Sciences, University of Bradford , Bradford, , UK; 2. Department of Dermatology, University Hospital Eppendorf, University of Hamburg, Hamburg, , DE;

 

ABSTRACT BODY: Abstract Body: The recent expansion of hair research activity owes much to the elegant microdissection and implantation experiments of Cohen and Oliver in the 1960's that confirmed a crucial role for the follicular/dermal papilla in hair growth. Study of the follicular papilla's involvement in hair growth regulation was greatly facilitated by the isolation and cultivation of this tiny cluster of fibroblast-like cells by Jahoda & Oliver in the rat vibrissae and by Messenger in the human hair follicle. While isolation of the follicular papilla from the former is relatively straight-forward, the current method to isolate the much smaller human hair follicle HF requires significant skill. Thus, the routine initiation of primary cultures of human scalp follicular papilla cells requires significant training, time and commitment. In an attempt to simply our follicular papilla cell culture methodology for new laboratory personnel, we have made significant refinements to the current method. Our method requires only two simple manipulations to isolate follicular papilla from intact isolated hair follicles. This very rapid and easy method isolates clean and intact follicular papillae. Together with their attachment via scratching to the growth surface, the isolation and cultivation of this important hair follicle component can now be achieved easily by the laboratory newcomer. The method relies for its simplicity on the removal of the follicular papilla from the outside of the intact hair follicle rather than via internal manipulations within the hair follicle itself.